Regulatory
Part:BBa_K1913008:Design
Designed by: Carina Nieuwenweg Group: iGEM16_Wageningen_UR (2016-10-13)
Vitamin B12 riboswitch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 199
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to use a riboswitch, the chassis should have receptors on the outside for the ligand.
Source
The riboswitch has been cloned from the btuB genes of Escherichia coli .